Composite

Part:BBa_K3924046:Design

Designed by: Wenda Zhang   Group: iGEM21_Tsinghua   (2021-10-21)


mchC-mchD polycistrons with Ptac lacO promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1830
    Illegal XbaI site found at 1017
    Illegal PstI site found at 126
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1830
    Illegal PstI site found at 126
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1830
    Illegal BglII site found at 417
    Illegal BglII site found at 580
    Illegal BamHI site found at 1823
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1830
    Illegal XbaI site found at 1017
    Illegal PstI site found at 126
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1830
    Illegal XbaI site found at 1017
    Illegal PstI site found at 126
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 464
    Illegal SapI.rc site found at 1384


Design Notes

mchC-mchD polycistrons with Ptac lacO promoter


Source

mchC-mchD polycistrons with Ptac lacO promoter

References